diff --git a/spyder/plugins/pythonpath/tests/test_utils.py b/spyder/plugins/pythonpath/tests/test_utils.py
index f4c1bdecc82..52c5baf0c23 100644
--- a/spyder/plugins/pythonpath/tests/test_utils.py
+++ b/spyder/plugins/pythonpath/tests/test_utils.py
@@ -28,6 +28,7 @@ def test_check_path(tmp_path):
         assert not check_path('lib\\dist-packages')
         assert not check_path('Lib/site-packages')
         assert not check_path('Anaconda3/pkgs')
+        assert not check_path("C:\\Users\\user\\AppData\\Roaming\\Python\\Scripts")
     else:
         # One digit Python versions
         assert not check_path('lib/python3.9/site-packages')
diff --git a/spyder/plugins/pythonpath/utils.py b/spyder/plugins/pythonpath/utils.py
index 807e88b9176..e0f1e82ef11 100644
--- a/spyder/plugins/pythonpath/utils.py
+++ b/spyder/plugins/pythonpath/utils.py
@@ -22,9 +22,11 @@ def check_path(path):
     """
     pattern_string = r'(ana|mini|micro)(conda|mamba|forge)\d*(/base)*/pkgs'
     if os.name == 'nt':
+        path_appdata = os.getenv("APPDATA").replace('\\', '/')
         pattern_string = (
             f'.*({pattern_string}'
-            r'|(l|L)ib/(site|dist)-packages).*'
+            r'|(l|L)ib/(site|dist)-packages.*'
+            f'|(AppData|{path_appdata})/Roaming/Python).*'
         )
     else:
         pattern_string = (