diff --git a/src/Aspire.AppHost.Sdk/SDK/Sdk.in.props b/src/Aspire.AppHost.Sdk/SDK/Sdk.in.props
index 565f171c584..f89d4ebc91a 100644
--- a/src/Aspire.AppHost.Sdk/SDK/Sdk.in.props
+++ b/src/Aspire.AppHost.Sdk/SDK/Sdk.in.props
@@ -1,9 +1,20 @@
 <Project>
   <PropertyGroup>
     <AspireHostingSDKVersion>@VERSION@</AspireHostingSDKVersion>
-    <!-- Do not import the workload targets as this project is instead using
-    the Aspire.AppHost.Sdk -->
+    <!-- Do not import the workload targets as this project is instead using the Aspire.AppHost.Sdk -->
     <SkipAspireWorkloadManifest>true</SkipAspireWorkloadManifest>
     <IsAspireHost>true</IsAspireHost>
   </PropertyGroup>
+
+  <PropertyGroup Condition=" '$(FileBasedProgram)' == 'true' ">
+    <PublishAot>false</PublishAot>
+    <PublishTrimmed>false</PublishTrimmed>
+    <EnableAotAnalyzer>false</EnableAotAnalyzer>
+    <EnableTrimAnalyzer>false</EnableTrimAnalyzer>
+    <EnableSingleFileAnalyzer>false</EnableSingleFileAnalyzer>
+    <_ImportMicrosoftNETSdkFromAspireSdk Condition=" '$(UsingMicrosoftNETSdk)' == '' ">true</_ImportMicrosoftNETSdkFromAspireSdk>
+    <_ForceAspireFileBasedAppHostDefaults Condition=" '$(IsAspireHost)' == 'true' ">true</_ForceAspireFileBasedAppHostDefaults>
+  </PropertyGroup>
+
+  <Import Project="Sdk.props" Sdk="Microsoft.NET.Sdk" Condition=" '$(_ImportMicrosoftNETSdkFromAspireSdk)' == 'true' " />
 </Project>
\ No newline at end of file
diff --git a/src/Aspire.AppHost.Sdk/SDK/Sdk.in.targets b/src/Aspire.AppHost.Sdk/SDK/Sdk.in.targets
index f179dd8a145..28c70e499cc 100644
--- a/src/Aspire.AppHost.Sdk/SDK/Sdk.in.targets
+++ b/src/Aspire.AppHost.Sdk/SDK/Sdk.in.targets
@@ -6,6 +6,8 @@
   This means they cannot be overridden in the csproj, and may cause ordering issues, particularly StaticWebAssets.
   -->
 
+  <Import Project="Sdk.targets" Sdk="Microsoft.NET.Sdk" Condition="'$(_ImportMicrosoftNETSdkFromAspireSdk)' == 'true'" />
+
   <ItemGroup>
     <ProjectCapability Include="DynamicFileNesting" />
     <ProjectCapability Include="DynamicFileNestingEnabled" />
@@ -49,11 +51,40 @@
     <AspireRidToolExecutable>$(AspireRidToolDirectory)Aspire.RuntimeIdentifier.Tool.dll</AspireRidToolExecutable>
   </PropertyGroup>
 
+  <!-- If the AppHost is a file-based app, then force disable AOT and Trimming.
+       This can be removed once https://github.com/dotnet/sdk/pull/50885 is merged and flows through to .NET 10 SDK as the values set in Sdk.props will be sufficient. -->
+  <Target Name="DisablePublishAotForAspireHost" BeforeTargets="_ComputeToolPackInputsToProcessFrameworkReferences;_CollectTargetFrameworkForTelemetry;ProcessFrameworkReferences;Restore;CollectPackageReferences"
+          Condition="'$(_ForceAspireFileBasedAppHostDefaults)' == 'true'">
+    <PropertyGroup>
+      <PublishAot>false</PublishAot>
+      <PublishTrimmed>false</PublishTrimmed>
+      <EnableAotAnalyzer>false</EnableAotAnalyzer>
+      <EnableTrimAnalyzer>false</EnableTrimAnalyzer>
+      <EnableSingleFileAnalyzer>false</EnableSingleFileAnalyzer>
+    </PropertyGroup>
+    <ItemGroup>
+      <ProjectCapability Remove="NativeAOT;PublishAot;PublishTrimmed" />
+    </ItemGroup>
+  </Target>
+
+  <!-- If the AppHost is a file-based app, add an implicit reference to the Aspire.Hosting.AppHost package if not already referenced.
+       This is done here so that file-based apps only need to set the single #sdk: directive line to make them an Aspire AppHost.
+       This mechanism can be disabled by setting `SkipAddAspireDefaultReferences` to `true` -->
+  <Target Name="AddImplicitAspireAppHostPackage" BeforeTargets="Restore;CollectPackageReferences" Condition="'$(_ForceAspireFileBasedAppHostDefaults)' == 'true' and '$(SkipAddAspireDefaultReferences)' != 'true'">
+    <PropertyGroup>
+      <_ImplicitAppHostVersion>@VERSION@</_ImplicitAppHostVersion>
+    </PropertyGroup>
+    <ItemGroup>
+      <PackageReference Include="Aspire.Hosting.AppHost" Version="$(_ImplicitAppHostVersion)" IsImplicitlyDefined="true"
+                        Condition="'@(PackageReference->WithMetadataValue('Identity','Aspire.Hosting.AppHost'))' == ''" />
+    </ItemGroup>
+  </Target>
+
   <!-- This target extracts the version of Aspire.Hosting.AppHost referenced by the project, and adds
        a reference to Aspire.Dashboard.Sdk and Aspire.Hosting.Orchestration for the build-time platform using
        the same version. This is done here dynamically to avoid having to pull in DCP and Dashboard packages
        for all of the platforms. This mechanism can be disabled by setting `SkipAddAspireDefaultReferences` to `true` -->
-  <Target Name="AddReferenceToDashboardAndDCP" BeforeTargets="Restore;CollectPackageReferences" Condition="'$(SkipAddAspireDefaultReferences)' != 'true'">
+  <Target Name="AddReferenceToDashboardAndDCP" BeforeTargets="Restore;CollectPackageReferences" AfterTargets="AddImplicitAspireAppHostPackage" Condition="'$(SkipAddAspireDefaultReferences)' != 'true'">
 
     <!-- First, we assume project is not using Central Package Management, and we try to extract the version
          from PackageReference Items. -->
diff --git a/src/Aspire.ProjectTemplates/templates/aspire-apphost-singlefile/9.5/apphost.cs b/src/Aspire.ProjectTemplates/templates/aspire-apphost-singlefile/9.5/apphost.cs
index 4c21e6630f6..d56ce4cf62c 100644
--- a/src/Aspire.ProjectTemplates/templates/aspire-apphost-singlefile/9.5/apphost.cs
+++ b/src/Aspire.ProjectTemplates/templates/aspire-apphost-singlefile/9.5/apphost.cs
@@ -1,7 +1,4 @@
-#:sdk Microsoft.NET.Sdk
 #:sdk Aspire.AppHost.Sdk@!!REPLACE_WITH_LATEST_VERSION!!
-#:package Aspire.Hosting.AppHost@!!REPLACE_WITH_LATEST_VERSION!!
-#:property PublishAot=false
 
 var builder = DistributedApplication.CreateBuilder(args);